The MDC party alleges that the vote result was rigged and President Emmerson Mnangagwa's narrow victory was due to a falsified vote count in Zimbabwe's first election since the end of Robert Mugabe's rule.
Chamisa is convinced that he won the popular vote ahead of the Zanu-PF leader who, according to ZEC, scrapped through with a slim margin of 50.8 percent to the plaintiff's 44.3 percent.
Zanu-PF Secretary for Administration, Cde Obert Mpofu, has stated that the MDC Alliance exposed its hypocrisy in the just ended elections by wanting to win at all costs yet any race produces a victor and a loser.
The matter is expected to be heard within 14 days as per the constitutional provisions.
Some of the lawyers who filed the papers for Chamisa include, Chris Mhike, Douglas Coltart, Advocate Thabani Mpofu, Advocate Dali Mpofu and Advocate Sylvester Hashiti.
Opposition Movement for Democratic Change (MDC) leader Nelson Chamisa addresses a media conference following the announcement of election results in Harare, Zimbabwe, August 3, 2018. "We have a good case and cause".More news: Dravidian icon M Karunanidhi passes away at the age of 94
The country's electoral laws allow challengers seven days to contest election results. "We are seeking a declaration to the effect that the presidential election was not properly conducted", MDC party lawyer Thanbani Mpofu said.
"If [the judges] think the evidence is weak they could throw the case out fairly fast".
On August 1, soldiers opened fire on MDC protesters, killing six people and sparking an global outcry.
The MDC arrived at the court less than an hour before it closed on Friday, the last day that the petition could be lodged.
Diplomats and election observers were present at the hearing in Harare after Biti fled to Zambia but was handed back to Zimbabwean police despite claiming asylum.
The 40-year-old opposition chief is strongly disputing the results announced by the Zimbabwe Electoral Commission and has vowed to go the "legal and constitutional" route to force the reversal of the outcome.